Recent Publications (2003- present)

Torkamani A, Kannan N, Taylor SS , Schork NJ. Congenital disease SNPs target lineage specific structural elements in protein kinases. Proc Natl Acad Sci U S A. 105(26):9011-6 (2008). Pubmed pdf

Kannan N, Taylor SS . Rethinking pseudokinases. Cell. 133(2):204-5 (2008). Pubmed pdf

Kornev AP, Taylor SS, Ten Eyck LF. A generalized allosteric mechanism for cis-regulated cyclic nucleotide binding domains. PLoS Comput Biol. 4(4):e1000056 (2008). Pubmed pdf

Ma Y, Taylor SS. A molecular switch for targeting between ER and mitochondria:Conversion of a mitochondria targeting element into an ER targeting signal in dAKAP1. J Biol Chem. 283(17):11743-51 (2008). Pubmed pdf

Masterson LR, Mascioni A, Traaseth NJ, Taylor SS, Veglia G. Allosteric cooperativity in protein kinase A. Proc Natl Acad Sci U S A. 105(2):506-11 (2008). Pubmed pdf

Kannan N, Wu J, Anand GS, Yooseph S, Neuwald AF, Venter CJ, Taylor SS. Evolution of allostery in the cyclic nucleotide binding module. Genome Biol. 8(12):R264 (2007). Pubmed pdf

Ten Eyck LF, Taylor SS, Kornev AP. Conserved spatial patterns across the protein kinase family. Biochim Biophys Acta. 1784(1):238-243 (2007). Pubmed pdf

Taylor SS, Kim C, Cheng CY, Brown SH, Wu J, Kannan N. Signaling through cAMP and cAMP-dependent protein kinase: Diverse strategies for drug design. Biochim Biophys Acta. 1784(1):16-26 (2007). Pubmed pdf

Valiev M, Yang J, Adams JA,Taylor SS, Weare JH. Phosphorylation Reaction in cAPK Protein Kinase-Free Energy Quantum Mechanical/Molecular Mechanics Simulations. J Phys Chem B. 111(47):13455-64. (2007). Pubmed pdf

Kannan N, Neuwald AF,Taylor SS. Analogous regulatory sites within the alphaC-beta4 loop regions of ZAP-70 tyrosine kinase and AGC kinases. Biochim Biophys Acta. 1784(1):27-32 (2007). Pubmed pdf

Anand GS, Hotchko M, Brown SH, Ten Eyck LF, Komives EA,Taylor SS. R-subunit Isoform Specificity in Protein Kinase A: Distinct Features of Protein Interfaces in PKA Types I and II by Amide H/(2)H Exchange Mass Spectrometry. J Mol Biol. 374(2):487-99 (2007). Pubmed pdf

Wu J, Brown SH, von Daake S, Taylor SS. PKA type IIalpha holoenzyme reveals a combinatorial strategy for isoform diversity. Science. 318(5848):274-9 (2007). Pubmed pdf

Alto NM, Weflen AW, Rardin MJ, Yarar D, Lazar CS, Tonikian R, Koller A,Taylor SS, Boone C, Sidhu SS, Schmid SL, Hecht GA, Dixon JE. The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways. J Cell Biol. 178(7):1265-78 (2007). Pubmed pdf

Kim C, Cheng CY, Saldanha SA, Taylor SS. PKA-I Holoenzyme Structure Reveals a Mechanism for cAMP-Dependent Activation. Cell. 130(6):1032-43 (2007). Pubmed pdf

Martin BR, Deerinck TJ, Ellisman MH, Taylor SS,Tsien RY. Isoform-Specific PKA Dynamics Revealed by Dye-Triggered Aggregation and DAKAP1alpha-Mediated Localization in Living Cells. Chem Biol. 14(9):1031-42 (2007). Pubmed pdf

Anand G, Taylor SS,Johnson DA. Cyclic-AMP and Pseudosubstrate Effects on Type-I A-Kinase Regulatory and Catalytic Subunit Binding Kinetics. Biochemistry. 46(32):9283-91 (2007). Pubmed pdf

Humphries KM, Pennypacker JK, Taylor SS. Redox regulation of PKA signaling: Kinase versus phosphatase inactivation. J Biol Chem. 282(30):22072-9 (2007). Pubmed pdf

Navarro L, Koller A, Nordfelth R, Wolf-Watz H, Taylor S, Dixon JE. Identification of a molecular target for the Yersinia protein kinase A. Mol Cell. 26(4):465-77 (2007). Pubmed pdf

Lim CJ, Han J, Yousefi N, Ma Y, Amieux PS, McKnight GS,Taylor SS,Ginsberg MH. alpha4 Integrins are Type I cAMP-dependent protein kinase-anchoring proteins. Nat Cell Biol. 9(4):415-21 (2007). Pubmed pdf

Kannan N,Taylor SS, Zhai Y, Venter JC, Manning G. Structural and Functional Diversity of the Microbial Kinome. PLoS Biol. 5(3):e17 (2007). Pubmed pdf

Yooseph S, Sutton G, Rusch DB, Halpern AL, Williamson SJ, Remington K, Eisen JA, Heidelberg KB, Manning G, Li W, Jaroszewski L, Cieplak P, Miller CS, Li H, Mashiyama ST, Joachimiak MP, van Belle C, Chandonia JM, Soergel DA, Zhai Y, Natarajan K, Lee S, Raphael BJ, Bafna V, Friedman R, Brenner SE, Godzik A, Eisenberg D, Dixon JE, Taylor SS, Strausberg RL, Frazier M, Venter JC. The Sorcerer II Global Ocean Sampling Expedition: Expanding the Universe of Protein Families. PLoS Biol. 5(3):e16 (2007). Pubmed pdf

Schauble S, King CC, Darshi M, Koller A, Shah K, Taylor SS. Identification of ChChd3 as a novel substrate of PKA using an analog-sensitive catalytic subunit. J Biol Chem. 282(20):14952-9 (2007). Pubmed pdf

Kannan N, Haste N, Taylor SS, Neuwald AF. The hallmark of AGC kinase functional divergence is its C-terminal tail, a cis-acting regulatory module. Proc Natl Acad Sci U S A. 104(4):1272-7 (2007). Pubmed pdf

Das R, Esposito V, Abu-Abed M, Anand GS, Taylor SS, Melacini G. cAMP activation of PKA defines an ancient signaling mechanism. Proc Natl Acad Sci U S A. 104(1):93-8 (2006). Pubmed pdf

Saldanha SA, Kaler G, Cottam HB, Abagyan R, Taylor SS. Assay principle for modulators of protein-protein interactions and its application to non-ATP-competitive ligands targeting protein kinase A. Anal Chem. 78(24):8265-72 (2006). Pubmed pdf

Kornev AP, Haste NM, Taylor SS, Eyck LF. Surface comparison of active and inactive protein kinases identifies a conserved activation mechanism. Proc Natl Acad Sci U S A. 103(47):17783-8 (2006). Pubmed pdf

Kinderman FS, Kim C, von Daake S, Ma Y, Pham BQ, Spraggon G, Xuong NH, Jennings PA, Taylor SS. A dynamic mechanism for AKAP binding to RII isoforms of cAMP-dependent protein kinase. Mol Cell. 24(3):397-408 (2006). Pubmed pdf

Cranston AN, Carniti C, Oakhill K, Radzio-Andzelm E, Stone EA, McCallion AS, Hodgson S, Clarke S, Mondellini P, Leyland J, Pierotti MA, Whittaker J, Taylor SS, Bongarzone I, Ponder BA. RET is constitutively activated by novel tandem mutations that alter the active site resulting in multiple endocrine neoplasia type 2B. Cancer Res. 66(20):10179-87 (2006). Pubmed pdf

Esposito V, Sjoberg T, Das R, Brown S, Taylor SS, Melacini G. NMR assignment of the cAMP-binding domain A of the PKA regulatory subunit. J Biomol NMR. 36 Suppl 1:64 (2006). Pubmed pdf

Kim C, Vigil D, Anand G, Taylor SS. Structure and dynamics of PKA signaling proteins. Eur J Cell Biol. 85(7):651-4 (2006). Pubmed pdf

Vigil D, Blumenthal DK, Taylor SS, Trewhella J. Solution Scattering Reveals Large Differences in the Global Structures of Type II Protein Kinase A Isoforms. J Mol Biol. 357(3):880-9 (2006). Pubmed pdf

Niedner RH, Buzko OV, Haste NM, Taylor A, Gribskov M,Taylor SS. Protein kinase resource: an integrated environment for phosphorylation research. Proteins. 63(1):78-86 (2006). Pubmed pdf

Gullingsrud J, Kim C,Taylor SS, McCammon JA. Dynamic binding of PKA regulatory subunit RIalpha. Structure. 14(1):141-9 (2006). Pubmed pdf

Vigil D, Lin JH, Sotriffer CA, Pennypacker JK, McCammon JA,Taylor SS. A simple electrostatic switch important in the activation of type I protein kinase A by cyclic AMP. Protein Sci. 15(1):113-21 (2006). Pubmed pdf

Burns-Hamuro LL, Hamuro Y, Kim JS, Sigala P, Fayos R, Stranz DD, Jennings PA, Taylor SS, Woods VL Jr. Distinct interaction modes of an AKAP bound to two regulatory subunit isoforms of protein kinase A revealed by amide hydrogen/deuterium exchange. Protein Sci. 14(12):2982-92 (2005). Pubmed pdf

Wu J, Yang J, Kannan N, Madhusudan, Xuong NH, Ten Eyck LF,Taylor SS. Crystal structure of the E230Q mutant of cAMP-dependent protein kinase reveals an unexpected apoenzyme conformation and an extended N-terminal A helix. Protein Sci. 14(11):2871-9 (2005). Pubmed pdf

Taylor SS, Kim C, Vigil D, Haste NM, Yang J, Wu J, Anand GS. Dynamics of signaling by PKA. Biochim Biophys Acta. 1754(1-2):25-37 (2005). Pubmed pdf

Taylor SS, Haste NM, Ghosh G. PKR and eIF2alpha: Integration of Kinase Dimerization, Activation, and Substrate Docking. Cell. 122(6):823-5 (2005). Pubmed pdf

Zhang J, Hupfeld CJ, Taylor SS, Olefsky JM, Tsien RY. Insulin disrupts beta-adrenergic signalling to protein kinase A in adipocytes. Nature. 437(7058):569-73 (2005). Pubmed pdf

Vigil D, Blumenthal DK, Taylor SS, Trewhella J. The conformationally dynamic C helix of the RIalpha -subunit of protein Kinase A mediates isoform specific domain reorganization upon C subunit binding. J Biol Chem. 280(42):35521-7 (2005). Pubmed pdf

Iyer GH, Garrod S, Woods VL Jr, Taylor SS. Catalytic independent functions of a protein kinase as revealed by a kinae-dead mutant: study of the Lys72His mutant of cAMP-dependent kinase. J Mol Biol. 351(5):1110-22 (2005). Pubmed pdf

Kim C, Xuong NH, Taylor SS. Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA. Science. 307(5710):690-6 (2005). Pubmed pdf

Yang J, Garrod SM, Deal MS, Anand GS, Woods VL Jr,Taylor SS. Allosteric network of cAMP-dependent protein kinase revealed by mutation of Tyr204 in the P+1 loop. J Mol Biol. 346(1):191-201 (2005). Pubmed pdf

Sastri M, Barraclough DM, Carmicheal PT, Taylor SS. A-kinase-interacting protein localizes protein kinase A in the nucleus. Proc Natl Acad Sci USA. 102(2):349-54 (2005). Pubmed pdf

Berman HM, Ten Eyck LF, Goodsell DS, Haste NM, Kornev A, Taylor SS. The cAMP binding domain: an ancient signaling module. Proc Natl Acad Sci USA. 102(1):45-50 (2005). Pubmed pdf

Iyer GH, Moore MJ, Taylor SS. Consequences of lysine 72 mutation on the phosphorylation and activation state of cAMP-dependent kinase. J Biol Chem. 280(10):8800-7 (2005). Pubmed pdf

Humphries KM, Deal MS, Taylor SS. Enhanced dephosphorylation of cAMP-dependent protein kinase by oxidation and thiol modification. J Biol Chem. 280(4):2750-8 (2005). Pubmed pdf

Burns-Hamuro LL, Barraclough DM, Taylor SS. Identification and functional analysis of dual-specific A kinase-anchoring protein-2. Methods Enzymol. 390:354-74 (2004). Pubmed pdf

Nolen B, Taylor SS, Ghosh G. Regulation of protein kinases; controlling activity through activation segment conformation. Mol Cell. (Camb). 15(5):661-75(2004). Pubmed pdf

Wu J, Brown S, Xuong NH, Taylor SS. RIa Subunit of PKA: A cAMP-free Structure Reveals a Hydrophobic Capping Mechanism for Docking cAMP into Site B. Structure. (Camb). 12(6):1057-65 (2004). Pubmed pdf

Vigil D, Blumenthal DK, Brown S, Taylor SS, Trewhella J. Differential Effect of Substrate Binding on TypeI and TypeII PKA Holoenzyme Dissociation. Biochemistry 43(19):5629-36 (2004). Pubmed pdf

Hamuro Y, Anand GS, Kim JS, Juliano C, Stranz DD, Taylor SS, and Woods VL Jr. Mapping Intersubunit Interactions of the Regulatory Subunit (RIa) in the Type I Holoenzyme of Protein Kinase A by Enhanced Amide Hydrogen/Deuterium Exchange Mass Spectrometry (DXMS). J. Mol. Biol. 340(5):1185-96 (2004). Pubmed pdf

Heller WT, Vigil D, Brown S, Blumenthal DK, Taylor SS, Trewhella, J. C subunits Binding to the Protein Kinase RIa Dimer Induces a Large Conformational Change. J. Biol. Chem. 279:19084-19090 (2004). Pubmed pdf

Vigil D, Blumenthal DK, Heller WT, Brown S, Canaves JM, Taylor SS, Trewhella J. Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regions. J. Mol. Biol. 337(5):1183-94 (2004) Pubmed pdf

Wu J, Jones JM, Nguyen-Huu X, Ten Eyck LF, Taylor SS. Crystal structures of RIalpha subunit of cyclic adenosine 5'-monophosphate (cAMP)-dependent protein kinase complexed with (Rp)-adenosine 3',5'-cyclic monophosphothioate and (Sp)-adenosine 3',5'-cyclic monophosphothioate, the phosphothioate analogues of cAMP. Biochemistry 43(21):6620-9 (2004). Pubmed pdf

Taylor SS, Yang J, Wu J, Haste NM, Radzio-Andzelm E, Anand G. PKA: a portrait of protein kinase dynamics. Biochim Biophys Acta.1697(1-2):259-69 (2004). Pubmed pdf

Yang J, Ten Eyck LF, Xuong NH, Taylor SS. Crystal structure of a cAMP-dependent protein kinase mutant at 1.26A: new insights into the catalytic mechanism. J Mol Biol. 336(2):473-87 (2004). Pubmed pdf

Akamine P, Madhusudan, Brunton LL, Ou HD, Canaves JM, Xuong NH, Taylor SS. Balanol analogues probe specificity determinants and the conformational malleability of the cyclic 3',5'-adenosine monophosphate-dependent protein kinase catalytic subunit. Biochemistry 43(1):85-96 (2004). Pubmed pdf

Zawadzki KM, Taylor SS. cAMP-dependent protein kinase regulatory subunit type IIbeta: active site mutations define an isoform-specific network for allosteric signaling by cAMP. J Biol Chem. 279(8):7029-36 (2004). Pubmed pdf

Anand GS, Law D, Mandell JG, Snead AN, Tsigelny I, Taylor SS, Ten Eyck LF, Komives EA. Identification of the protein kinase A regulatory RIalpha-catalytic subunit interface by amide H/2H exchange and protein docking. Proc Natl Acad Sci U S A. 100(23):13264-9 (2003). Pubmed pdf

Gisler SM, Madjdpour C, Bacic D, Pribanic S, Taylor SS, Biber J, Murer H. PDZK1: II. an anchoring site for the PKA-binding protein D-AKAP2 in renal proximal tubular cells. Kidney Int. 64(5):1746-54 (2003). Pubmed pdf

Zawadzki KM, Hamuro Y, Kim JS, Garrod S, Stranz DD, Taylor SS, Woods VL Jr. Dissecting interdomain communication within cAPK regulatory subunit type IIbeta using enhanced amide hydrogen/deuterium exchange mass spectrometry (DXMS). Protein Sci. 12(9):1980-90 (2003). Pubmed pdf

Banky P, Roy M, Newlon MG, Morikis D, Haste NM, Taylor SS, Jennings PA. Related protein-protein interaction modules present drastically different surface topographies despite a conserved helical platform. J Mol Biol. 330(5):1117-29 (2003). Pubmed pdf

Zawadzki KM, Pan CP, Barkley MD, Johnson D, Taylor SS. Endogenous tryptophan residues of cAPK regulatory subunit type IIbeta reveal local variations in environments and dynamics. Proteins. 51(4):552-61 (2003). Pubmed pdf

Burns LL, Canaves JM, Pennypacker JK, Blumenthal DK, Taylor SS. Isoform specific differences in binding of a dual-specificity A-kinase anchoring protein to type I and type II regulatory subunits of PKA. Biochemistry 42(19):5754-63 (2003). Pubmed pdf

Hamuro Y, Zawadzki KM, Kim JS, Stranz DD, Taylor SS, Woods VL Jr. Dynamics of cAPK type IIbeta activation revealed by enhanced amide H/2H exchange mass spectrometry (DXMS). J Mol Biol. 327(5):1065-76 (2003) Pubmed pdf

Kammerer S, Burns-Hamuro LL, Ma Y, Hamon SC, Canaves JM, Shi MM, Nelson MR, Sing CF, Cantor CR, Taylor SS, Braun A. Amino acid variant in the kinase binding domain of dual-specific A kinase-anchoring protein 2: a disease susceptibility polymorphism. Proc Natl Acad Sci U S A. 100(7):4066-71 (2003). Pubmed pdf

Burns-Hamuro LL, Ma Y, Kammerer S, Reineke U, Self C, Cook C, Olson GL, Cantor CR, Braun A, Taylor SS. Designing isoform-specific peptide disruptors of protein kinase A localization. Proc Natl Acad Sci U S A. 100(7):4072-7 (2003). Pubmed pdf

Akamine P, Madhusudan, Wu J, Xuong NH, Ten Eyck LF, Taylor SS. Dynamic features of cAMP-dependent protein kinase revealed by apoenzyme crystal structure. J Mol Biol. 327(1):159-71 (2003). Pubmed pdf

Moore MJ, Adams JA, Taylor SS. Structural basis for peptide binding in protein kinase A. Role of glutamic acid 203 and tyrosine 204 in the peptide-positioning loop. J Biol Chem. 278(12):10613-8 (2003). Pubmed pdf

Selected Publications (1980-2002)

Moore MJ, Kanter JR, Jones KC, Taylor SS. Phosphorylation of the catalytic subunit of protein kinase A. Autophosphorylation versus phosphorylation by phosphoinositide-dependent kinase-1. J Biol Chem. 277(49):47878-84(2002). Pubmed pdf

Hamuro Y, Burns L, Canaves J, Hoffman R, Taylor SS, Woods V. Domain organization of D-AKAP2 revealed by enhanced deuterium exchange-mass spectrometry (DXMS). J Mol Biol. 321(4):703-14(2002). Pubmed pdf

Ma Y, Taylor SS. A 15-residue bifunctional element in D-AKAP1 is required for both endoplasmic reticulum and mitochondrial targeting. J Biol Chem. 277(30):27328-36(2002). Pubmed pdf

Madhusudan, Akamine P, Xuong NH, Taylor SS. Crystal structure of a transition state mimic of the catalytic subunit of cAMP-dependent protein kinase. Nat Struct Biol. 9(4):273-7(2002). Pubmed pdf

Li F, Gangal M, Juliano C, Gorfain E, Taylor SS, Johnson DA. Evidence for an internal entropy contribution to phosphoryl transfer: a study of domain closure, backbone flexibility, and the catalytic cycle of cAMP-dependent protein kinase. J Mol Biol.315(3):459-69(2002). Pubmed pdf

Zhang J, Ma Y, Taylor SS, Tsien RY. Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering. Proc Natl Acad Sci U S A.98(26):14997-5002(2001). Pubmed pdf

Johnson DA, Akamine P, Radzio-Andzelm E, Madhusudan M, Taylor SS. Dynamics of cAMP-dependent protein kinase. Chem Rev.101(8):2243-70(Invited paper 2001). Pubmed pdf

Canaves JM, Taylor SS. Classification and phylogenetic analysis of the cAMP-dependent protein kinase regulatory subunit family. J Mol Evol.54(1):17-29(2002). Pubmed pdf

Wang L, Sunahara RK, Krumins A, Perkins G, Crochiere ML, Mackey M, Bell S, Ellisman MH, Taylor SS. Cloning and mitochondrial localization of full-length D-AKAP2, a protein kinase A anchoring protein. Proc Natl Acad Sci U S A.98(6):3220-5(2001). Pubmed pdf

Diller TC, Madhusudan, Xuong NH, Taylor SS. Molecular basis for regulatory subunit diversity in cAMP-dependent protein kinase: crystal structure of the type II beta regulatory subunit. Structure.9(1):73-82(2001). Pubmed pdf

Li F, Gangal M, Jones JM, Deich J, Lovett KE, Taylor SS, Johnson DA. Consequences of cAMP and catalytic-subunit binding on the flexibility of the A-kinase regulatory subunit. Biochemistry.96(22):12394-9(2000). Pubmed pdf

Banky P, Newlon MG, Roy M, Garrod S, Taylor SS, Jennings PA. Isoform-specific differences between the type Ialpha and IIalpha cyclic AMP-dependent protein kinase anchoring domains revealed by solution NMR. J Biol Chem. 275(45):35146-52(2000). Pubmed pdf

Aimes RT, Hemmer W, Taylor SS. Serine-53 at the tip of the glycine-rich loop of cAMP-dependent protein kinase: role in catalysis, P-site specificity, and interaction with inhibitors. Biochemistry. 39(28):8325-32(2000). Pubmed pdf

Gangal M, Clifford T, Deich J, Cheng X, Taylor SS, Johnson DA. Mobilization of the A-kinase N-myristate through an isoform-specific intermolecular switch. Proc Natl Acad Sci U S A.39(50):15626-32(1999). Pubmed pdf

Tsigelny I, Greenberg JP, Cox S, Nichols WL, Taylor SS, Ten Eyck LF. 600 ps molecular dynamics reveals stable substructures and flexible hinge points in cAMP dependent protein kinase. Biopolymers. 50(5):513-24(1999). Pubmed pdf

Huang LJ, Wang L, Ma Y, Durick K, Perkins G, Deerinck TJ, Ellisman MH, Taylor SS. NH2-Terminal targeting motifs direct dual specificity A-kinase-anchoring protein 1 (D-AKAP1) to either mitochondria or endoplasmic reticulum. J Cell Biol. 145(5):951-9(1999). Pubmed pdf

Hauer JA, Taylor SS, Johnson DA. Binding-dependent disorder-order transition in PKI alpha: a fluorescence anisotropy study. Biochemistry. 38(21):6774-80(1999). Pubmed pdf

Herberg FW, Doyle ML, Cox S, Taylor SS. Dissection of the nucleotide and metal-phosphate binding sites in cAMP-dependent protein kinase. Biochemistry. 38(19):6352-60(1999). Pubmed pdf

Hauer JA, Barthe P, Taylor SS, Parello J, Padilla A. Two well-defined motifs in the cAMP-dependent protein kinase inhibitor (PKIalpha) correlate with inhibitory and nuclear export function. Protein Sci. 8(3):545-53(1999). Pubmed pdf

Narayana N, Diller TC, Koide K, Bunnage ME, Nicolaou KC, Brunton LL, Xuong NH, Ten Eyck LF, Taylor SS. Crystal structure of the potent natural product inhibitor balanol in complex with the catalytic subunit of cAMP-dependent protein kinase. Biochemistry. 38(8):2367-76(1999). Pubmed pdf

Banky P, Huang LJ, Taylor SS. Dimerization/docking domain of the type Ialpha regulatory subunit of cAMP-dependent protein kinase. Requirements for dimerization and docking are distinct but overlapping. J Biol Chem. 273(52):35048-55(1998). Pubmed pdf

Struppe J, Komives EA, Taylor SS, Vold RR. 2H NMR studies of a myristoylated peptide in neutral and acidic phospholipid bicelles. Biochemistry. 37(44):15523-7(1998). Pubmed pdf

Huang LJ, Taylor SS. Dissecting cAMP binding domain A in the RIalpha subunit of cAMP-dependent protein kinase. Distinct subsites for recognition of cAMP and the catalytic subunit. J Biol Chem. 273(41):26739-46(1998). Pubmed pdf

Cheng X, Ma Y, Moore M, Hemmings BA, Taylor SS. Phosphorylation and activation of cAMP-dependent protein kinase by phosphoinositide-dependent protein kinase. Proc Natl Acad Sci U S A. 95(17):9849-54(1998). Pubmed pdf

Grant BD, Hemmer W, Tsigelny I, Adams JA, Taylor SS. Kinetic analyses of mutations in the glycine-rich loop of cAMP-dependent protein kinase. Biochemistry. 37(21):7708-15(1998). Pubmed pdf

Shaltiel S, Cox S, Taylor SS. Conserved water molecules contribute to the extensive network of interactions at the active site of protein kinase A. Proc Natl Acad Sci U S A. 95(2):484-91(1998). Pubmed pdf

Gibson RM, Taylor SS. Dissecting the cooperative reassociation of the regulatory and catalytic subunits of cAMP-dependent protein kinase. Role of Trp-196 in the catalytic subunit. J Biol Chem. 272(51):31998-2005(1997). Pubmed pdf

Leon DA, Herberg FW, Banky P, Taylor SS. A stable alpha-helical domain at the N terminus of the RIalpha subunits of cAMP-dependent protein kinase is a novel dimerization/docking motif. J Biol Chem. 272(45):28431-7(1997). Pubmed pdf

Huang LJ, Durick K, Weiner JA, Chun J, Taylor SS. D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain. Proc Natl Acad Sci U S A. 94(21):11184-9(1997). Pubmed pdf

Narayana N, Cox S, Nguyen-huu X, Ten Eyck LF, Taylor SS. A binary complex of the catalytic subunit of cAMP-dependent protein kinase and adenosine further defines conformational flexibility. Structure. 5(7):921-35(1997). Pubmed pdf

Gibson RM, Ji-Buechler Y, Taylor SS. Identification of electrostatic interaction sites between the regulatory and catalytic subunits of cyclic AMP-dependent protein kinase. Protein Sci. 6(9):1825-34(1997). Pubmed pdf

Gibson RM, Ji-Buechler Y, Taylor SS. Interaction of the regulatory and catalytic subunits of cAMP-dependent protein kinase. Electrostatic sites on the type Ialpha regulatory subunit. J Biol Chem. 272(26):16343-50(1997). Pubmed pdf

Hemmer W, McGlone M, Tsigelny I, Taylor SS. Role of the glycine triad in the ATP-binding site of cAMP-dependent protein kinase. J Biol Chem. 272(27):16946-54(1997). Pubmed pdf

Narayana N, Cox S, Shaltiel S, Taylor SS, Xuong N. Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine. Biochemistry. 36(15):4438-48(1997). Pubmed pdf

Huang LJ, Durick K, Weiner JA, Chun J, Taylor SS. Identification of a novel protein kinase A anchoring protein that binds both type I and type II regulatory subunits. J Biol Chem. 272(12):8057-64(1997). Pubmed pdf

Herberg FW, Zimmermann B, McGlone M, Taylor SS. Importance of the A-helix of the catalytic subunit of cAMP-dependent protein kinase for stability and for orienting subdomains at the cleft interface. Protein Sci. 6(3):569-79(1997). Pubmed pdf

Padilla A, Hauer JA, Tsigelny I, Parello J, Taylor SS. Solution structure of synthetic peptide inhibitor and substrate of cAMP-dependent protein kinase. A study by 2D H NMR and molecular dynamics. J Pept Res. 49(3):210-20(1997). Pubmed

Chestukhin A, Litovchick L, Schourov D, Cox S, Taylor SS, Shaltiel S. Functional malleability of the carboxyl-terminal tail in protein kinase A. J Biol Chem. 271(17):10175-82(1996). Pubmed pdf

Grant BD, Tsigelny I, Adams JA, Taylor SS. Examination of an active-site electrostatic node in the cAMP-dependent protein kinase catalytic subunit. Protein Sci. 5(7):1316-24(1996). Pubmed pdf

Tsigelny I, Grant BD, Taylor SS, Ten Eyck LF. Catalytic subunit of cAMP-dependent protein kinase: electrostatic features and peptide recognition. Biopolymers. 39(3):353-65(1996). Pubmed pdf

Herberg FW, Taylor SS, Dostmann WR. Active site mutations define the pathway for the cooperative activation of cAMP-dependent protein kinase. Biochemistry. 35(9):2934-42(1996). Pubmed pdf

Koide K, Bunnage ME, Gomez Paloma L, Kanter JR, Taylor SS, Radzio-Andzelm E, Hunter T. Molecular design and biological activity of potent and selective protein kinase inhibitors related to balanol. Chem Biol. 2(9):601-8(1995). Pubmed pdf

Taylor SS, Radzio-Andzelm E, Hunter T. How do protein kinases discriminate between serine/threonine and tyrosine? Structural insights from the insulin receptor protein-tyrosine kinase. FASEB J. 9(13):1255-66(1995). Pubmed pdf

Su Y, Dostmann WR, Herberg FW, Durick K, Xuong NH, Ten Eyck L, Taylor SS, Varughese KI. Regulatory subunit of protein kinase A: structure of deletion mutant with cAMP binding domains. Science. 269(5225):807-13(1995). Pubmed

Wen W, Meinkoth JL, Tsien RY, Taylor SS. Identification of a signal for rapid export of proteins from the nucleus. Cell. 82(3):463-73(1995). Pubmed pdf

Adams JA, McGlone ML, Gibson R,Taylor SS. Phosphorylation modulates catalytic function and regulation in the cAMP-dependent protein kinase. Biochemistry.34(8):2447-54(1995). Pubmed

Wen W, Taylor SS, Meinkoth JL. The expression and intracellular distribution of the heat-stable protein kinase inhibitor is cell cycle regulated. J Biol Chem.270(5):2041-6(1995). Pubmed pdf

Taylor SS, Radzio-Andzelm E. Three protein kinase structures define a common motif. Structure. 2(5):345-55(1994). Pubmed

Madhusudan, Trafny EA, Xuong NH, Adams JA, Ten Eyck LF, Taylor SS, Sowadski JM. cAMP-dependent protein kinase: crystallographic insights into substrate recognition and phosphotransfer. Protein Sci. 3(2):176-87(1994). Pubmed pdf

Wen W, Taylor SS. High affinity binding of the heat-stable protein kinase inhibitor to the catalytic subunit of cAMP-dependent protein kinase is selectively abolished by mutation of Arg133. J Biol Chem.269(11):8423-30(1994). Pubmed pdf

Herberg FW, Taylor SS. Physiological inhibitors of the catalytic subunit of cAMP-dependent protein kinase: effect of MgATP on protein-protein interactions. Biochemistry. 32(50):14015-22(1993). Pubmed

Adams JA, Taylor SS. Divalent metal ions influence catalysis and active-site accessibility in the cAMP-dependent protein kinase. Protein Sci. 2(12):2177-86(1993). Pubmed pdf

Zheng J, Knighton DR, Xuong NH, Taylor SS, Sowadski JM, Ten Eyck LF. Crystal structures of the myristylated catalytic subunit of cAMP-dependent protein kinase reveal open and closed conformations. Protein Sci. 2(10):1559-73(1993). Pubmed pdf

Karlsson R, Zheng J, Xuong N, Taylor SS, Sowadski JM. Structure of the mammalian catalytic subunit of cAMP-dependent protein kinase and an inhibitor peptide displays an open conformation. Acta Crystallogr D Biol Crystallogr. 49(Pt 4):381-8(1993). Pubmed pdf

Yonemoto W, Garrod SM, Bell SM, Taylor SS. Identification of phosphorylation sites in the recombinant catalytic subunit of cAMP-dependent protein kinase. J Biol Chem. 268(25):18626-32(1993). Pubmed pdf

Taylor SS, Zheng J, Radzio-Andzelm E, Knighton DR, Ten Eyck LF, Sowadski JM, Herberg FW, Yonemoto WM. cAMP-dependent protein kinase defines a family of enzymes. Philos Trans R Soc Lond B Biol Sci. 340(1293):315-24(1993). Pubmed pdf

Zheng J, Knighton DR, Ten Eyck LF, Karlsson R, Xuong N, Taylor SS, Sowadski JM. Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor. Biochemistry. 32(9):2154-61(1993). Pubmed

Adams JA, Taylor SS. Energetic limits of phosphotransfer in the catalytic subunit of cAMP-dependent protein kinase as measured by viscosity experiments. Biochemistry. 31(36):8516-22(1992). Pubmed

Knighton DR, Zheng JH, Ten Eyck LF, Xuong NH, Taylor SS, Sowadski JM. Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science. 253(5018):414-20(1991). Pubmed

Knighton DR, Zheng JH, Ten Eyck LF, Ashford VA, Xuong NH,Taylor SS, Sowadski JM. Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science. 253(5018):407-14(1991). Pubmed

Adams SR, Harootunian AT, Buechler YJ, Taylor SS, Tsien RY. Fluorescence ratio imaging of cyclic AMP in single cells. Biochemistry. 349(6311):694-7 (1991). Pubmed pdf

First EA, Taylor SS. Selective modification of the catalytic subunit of cAMP-dependent protein kinase with sulfhydryl-specific fluorescent probes. Biochemistry. 28(8):3598-605 (1989). Pubmed

First EA, Johnson DA,Taylor SS. Fluorescence energy transfer between cysteine 199 and cysteine 343: evidence for MgATP-dependent conformational change in the catalytic subunit of cAMP-dependent protein kinase. Biochemistry. 28(8):3606-13 (1989). Pubmed

Buechler JA,Taylor SS. Dicyclohexylcarbodiimide cross-links two conserved residues, Asp-184 and Lys-72, at the active site of the catalytic subunit of cAMP-dependent protein kinase. Biochemistry. 28(5):2065-70 (1989). Pubmed

Buechler JA,Taylor SS. Identification of aspartate-184 as an essential residue in the catalytic subunit of cAMP-dependent protein kinase. Biochemistry. 27(19):7356-61 (1988). Pubmed

Toner-Webb J, Taylor SS. Inhibition of the catalytic subunit of cAMP-dependent protein kinase by dicyclohexylcarbodiimide. Biochemistry. 26(23):7371-8 (1987). Pubmed

Weber IT, Steitz TA, Bubis J, Taylor SS. Predicted structures of cAMP binding domains of type I and II regulatory subunits of cAMP-dependent protein kinase. Biochemistry. 26(2):343-51 (1987). Pubmed

Bubis J, Taylor SS. Covalent modification of both cAMP binding sites in cAMP-dependent protein kinase I by 8-azidoadenosine 3',5'-monophosphate. Biochemistry. 24(9):2163-70 (1985). Pubmed

First EA, Taylor SS. Induced interchain disulfide bonding in cAMP-dependent protein kinase II. J Biol Chem. 259(7):4011-4 (1984). Pubmed pdf

Bramson HN, Thomas N, Matsueda R, Nelson NC,Taylor SS, Kaiser ET. Modification of the catalytic subunit of bovine heart cAMP-dependent protein kinase with affinity labels related to peptide substrates. J Biol Chem. 257(18):10575-81 (1982). Pubmed pdf

Zoller MJ, Nelson NC,Taylor SS. Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine. Covalent modification of lysine 71. J Biol Chem. 256(21):10837-42 (1981). Pubmed pdf

Kerlavage AR,Taylor SS. Covalent modification of an adenosine 3':5'-monophosphate binding site of the regulatory subunit of cAMP-dependent protein kinase II with 8-azidoadenosine 3':5'-monophosphate. Identification of a single modified tyrosine residue. J Biol Chem. 255(18):8483-8 (1980). Pubmed pdf